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food and cookingfermentationjapanmicrobiologySeptember 17, 20263 min read

What Turns Rice Into Sake, Miso and Soy Sauce? The Same Mould Every Time

By the BrainSnail editorial team. How these articles are written and checked, and how to tell us when one is wrong.

A domesticated mould grown deliberately on cooked grain supplies the enzymes that break starch and protein into sugars and savoury compounds, and it underpins a whole cuisine.

What it actually is

The material is cooked grain, usually rice or barley or soybeans, on which a particular mould has been grown under controlled warmth and humidity until the grains are covered in white fuzz and smell sweet and faintly of chestnuts. The organism is a species of Aspergillus that has been selected over centuries for safety and for enzyme production. The point of growing it is not the mould itself but what it secretes, and the finished material is essentially a convenient solid package of enzymes ready to be added to something else.

What the enzymes do

Two families of enzymes do almost all the work:

  • Amylases, which cut starch into simple sugars
  • Proteases, which cut proteins into peptides and amino acids
  • The sugars feed yeast and bacteria in later fermentation
  • The amino acids, especially glutamate, supply savoury depth
  • Lipases contribute aroma compounds in smaller amounts
  • Nothing else in the process performs either job

Why it changes what is possible

Making alcohol from grain requires converting starch to sugar first, because yeast cannot eat starch, and the world has two main solutions to that. European brewing germinates the grain so that the seed's own enzymes do the conversion, which is what malting is. East Asian practice grows the mould instead, and the crucial difference is that the mould works alongside the yeast in the same vessel at the same time, converting starch and fermenting the resulting sugar simultaneously. That parallel arrangement lets sake reach an alcohol content well above beer without distillation, which is unusual for a brewed drink.

Why it has to be watched

Growing a mould deliberately on warm damp grain is a controlled version of something that normally goes wrong, and the control is the skill. The grain is steamed to kill competitors, cooled to the right temperature and inoculated with spores, then spread in trays and turned by hand as the culture generates its own heat. Too hot and the enzyme balance shifts or the culture dies, too cool and unwanted organisms take hold, too wet and bacteria win. Traditional makers judged all of this by touch and smell, sleeping beside the room for two days, and the work is still described as the hardest part of the craft.

How it spread beyond Japan

The technique is ancient across East Asia and its recent history is a story of deliberate transfer. Chinese practice uses related preparations with mixed cultures, and the Japanese version is unusual in relying on a single purified strain, sold commercially as spores since the Edo period by specialist houses. In the last two decades Western chefs have adopted it for purposes it was never used for, growing it on unconventional substrates to produce rapid savoury seasonings, cured meats without long ageing and vegetable preparations with meaty depth. The appeal is that it delivers in days the amino acid content that traditional ageing takes months to develop.

The takeaway

Cooked grain covered in a domesticated Aspergillus is a solid package of enzymes, chiefly amylases that cut starch into sugar and proteases that cut protein into savoury amino acids. Because the mould works in the same vessel as the yeast, sake ferments and converts starch simultaneously and reaches strengths unusual for a brewed drink. Western kitchens now use it to reach aged flavours in days.

Practise this

Questions from Grains and Bread

Reading about something is not the same as being able to recall it. These are real questions from the Grains and Bread unit in our Food & Cooking track, answers and explanations included. The unit has 119 in total across 20 steps.

  • Build the sentenceLevel 3

    1. Build a sentence about fermentation.

    Answer: Yeast ferments sugars producing carbon dioxide and alcohol

    Yeast ferments sugars producing carbon dioxide and alcohol.

  • Multiple choiceLevel 2

    2. What is flour made by doing to grain?

    • Grinding it into powdercorrect
    • Boiling it
    • Freezing it
    • Drying it whole

    Grinding or milling crushes grain into a fine powder.

  • Multiple choiceLevel 2

    3. Which grain is used to make most bread?

    • Wheatcorrect
    • Rice
    • Barley
    • Millet

    Wheat contains the proteins that make stretchy bread dough.